Recombinant Rabbit Monoclonal Antibody to Trimethylated Histone H3 Lysine 27, H3K27me3 (Clone: RM175)(Discontinued)
Figure 1: Clone: RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2),or other methylations in Histone H3.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3. Western Blot: 1 µg/ml – 2 µg/ml; IHC: 0.5 µg/ml – 2 µg/ml; ELISA: 0.5 µg/ml - 1 µg/ml; Multiplex: 0.1 µg/ml – 0.5 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome |
| Post transnational modification: | Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis. |
| BioGrid: | 109273. 18 interactions. |
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