Recombinant Rabbit Monoclonal Antibody to Smad4 (Clone: RM277)(Discontinued)
Figure 1: Western Blot of Jurkat and IMR32 cells lysates using Anti-Smad4 Rabbit Monoclonal Antibody (Clone: RM277) at a 1:500 dilution.
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | Antibody in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM277 reacts to human Smad4. This antibody may also react to mouse or rat Smad4, as predicted by immunogen homology. Immunohistochemistry (IHC): 1:1000-1:2000 dilution; Western Blot (WB): 1:500-1:1000 dilution.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus |
| Post transnational modification: | Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling. |
| BioGrid: | 110264. 225 interactions. |
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