Recombinant Rabbit Monoclonal Antibody to Phosphothreonine (Clone: RM102)(Discontinued)
Figure 1: Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone: RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
| Immunogen Information : | Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide |
Clone RM102 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides. Western Blot (WB): 1:500 -1:2000 dilution; Immunoprecipitation (IP): 1:100-1:500 dilution; Chromatin IP (ChIP): 1:100-1:500 dilution; Immunocytochemistry (ICC): 1:100-1:500 dilution; Immunohistochemistry (IHC): 1:100-1:500 dilution.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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