Recombinant Rabbit Monoclonal Antibody to Phospho-EGFR (Y1173) (Clone: RM269)(Discontinued)
Figure 1: Western Blot of A431 cell nontreated (-) or treated (+) with EGF, using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (Clone: RM269) at a 1:500 dilution, showed Phospho-EGFR (Tyr1173) in EGF-treated A431 cells.
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | Antibody in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM269 reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173. Immunohistochemistry (IHC): 1:500-1:1000 dilution; Western Blot (WB): 1:1000-1:5000 dilution.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Secreted |
| Post transnational modification: | Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. |
| Tissue Specificity: | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. |
| BioGrid: | 108276. 1217 interactions. |
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