Recombinant Rabbit Monoclonal Antibody to His-Tag (6xHis-Tag or 10xHis-Tag) (Clone: RM146)(Discontinued)
Figure 1: Western blot of 293T cells: (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y Using Clone: RM146 at 0.2 µg/ml.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
| Immunogen Information : | Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus. |
Clone RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy teClone RMinus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. Western Blot: 0.1 µg/ml – 1 µg/ml; Immunoprecipitation: 0.5 µg/ml – 2 µg/ml; Immunocytochemistry 0.5 µg/ml – 2 µg/ml; Flow Cytometry: 0.5 µg/ml – 2 µg/ml; Immunohistochemistry; 0.1 µg/ml-1 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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