Recombinant Rabbit Monoclonal Antibody to Core Histone Macro H2A.1 (H2AFY) (Clone: RM248)(Discontinued)

Product code: 10-9587

Clone name : RM248
Clonality : Monoclonal
Application : ELISA, WB, Multiplex
Reactivity : Human

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Format : Purified
Amount : 100 µg
Isotype : Rabbit IgG
Purification : Protein A affinity purified from an animal origin-free culture supernatant
Content : 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Storage condition : Store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : H2afy
Gene ID : 26914
Uniprot ID : Q9QZQ8
Alternative Name : H2A/y, H2A/y
Immunogen Information : A peptide corresponding to the C-terminus of human Histone macroH2A1.

Clone RM248 reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross reactivity with other histone proteins.  Western Blot: 0.5  µg/ml - 2  µg/ml; ICC: 1 µg/ml - 2 µg/ml; ELISA: 0.2  µg/ml - 1  µg/ml; Multiplex: 0.2  µg/ml - 1  µg/ml.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus, Chromosome
Post transnational modification: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin (By similarity).
Tissue Specificity: Widely expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, expressed in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.
BioGrid: 205061. 2 interactions.
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