Recombinant Rabbit Monoclonal Antibody to Caspase-3 (Clone: RM250)(Discontinued)
Figure 1: Western Blot of Jurkat cell lysate, untreated or treated with etoposide, using Anti-Caspase-3 Clone: RM250 at a 1:1000 dilution.
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | Antibody in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM250 reacts to human full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa). Immunohistochemistry (IHC): 1:1000-1:2500 dilution; Western Blot (WB): 1:1000-1:2000 dilution.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Post transnational modification: | S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. |
| Tissue Specificity: | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. |
| BioGrid: | 107286. 102 interactions. |
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