Recombinant Rabbit Monoclonal Antibody to BRAF (V600E) Mutant (Clone: RM8)(Discontinued)
Figure 1: Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of Clone: RM8 to the V600E mutation. His-tag fusion proteins were captured by an Anti-His-Tag antibody.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
IHC, ICC: 2 – 10 µg/ml; ELISA: 0.5 – 2 µg/ml; WB: 0.5 – 2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Cytoplasm, Cell membrane |
| Post transnational modification: | Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
| Tissue Specificity: | Brain and testis. |
| BioGrid: | 107141. 63 interactions. |
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