Polyclonal Antibody to SHP-2 (Ab-542)

Figure 1: Western blot analysis of extracts from 3T3 cells using SHP-2(Ab-542) Antibody 35-1640 .

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Figure 1: Western blot analysis of extracts from 3T3 cells using SHP-2(Ab-542) Antibody 35-1640 .

Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2(Ab-542) Antibody 35-1640 (left) or the same antibody preincubated with blocking peptide(right).

Figure 3: Immunofluorescence staining of methanol-fixed Hela cells using SHP-2(Ab-542) Antibody 35-1640 .

Product code: 35-1640

Clonality :	Polyclonal
Application :	IHC, WB, IF
Reactivity :	Rat, Mouse, Human

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit IgG
Content :	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	PTPN11
Gene ID :	5781
Uniprot ID :	Q06124
Alternative Name :	Protein-tyrosine phosphatase 2C
Immunogen Information :	Peptide sequence around aa. 540~544 (H-E-Y-T-N) derived from Human SHP-2.

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14(9): 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96(17): 9677-9682 Manes S, et al. (1999) Mol Cell Biol ; 19(4): 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.

Predicted MW: 72kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Nucleus
Post transnational modification:	Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.
Tissue Specificity:	Widely expressed, with highest levels in heart, brain, and skeletal muscle.
BioGrid:	111745. 178 interactions.