Polyclonal Antibody to Microtubule Associated Protein t, MAPT

Name: Polyclonal Antibody to Microtubule Associated Protein t, MAPT
Brand: Abeomics
SKU: 34-1067
Price: 462.0000 USD
Availability: InStock
Rating: 4.7 (10 reviews)

Figure-1: Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-Ï„, (34-1067), dilution 1:2,000 in green, and costained with mouse mAb to MAP2,(34-1065), dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The (34-1067) antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.

Polyclonal Antibody to Microtubule Associated Protein t, MAPT

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Figure-1: Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-Ï„, (34-1067), dilution 1:2,000 in green, and costained with mouse mAb to MAP2,(34-1065), dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The (34-1067) antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.

Figure-2: Western blot analysis of tissue lysates using chicken pAb to MAP-Ï„,(34-1067), dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.

Product code: 34-1067

Clonality :	Polyclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human, Rat, Mouse, Cow, Pig, Horse

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Conc. IgY prep.
Amount :	100 µl
Isotype :	Chicken, IgY
Content :	Antibody is supplied as an aliquot of IgY preparation.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	MAPT
Gene ID :	4137
Uniprot ID :	P10636
Alternative Name :	Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau
Immunogen Information :	Recombinant full length version of the shortest human Tau isoform purified from E. coli.

WB: 1:10,000. IF/ICC and IHC: 1:1,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cell membrane, Cytoplasm, Cell projection, Cytoplasm, Cell projection
Post transnational modification:	Glycation of PHF-tau, but not normal brain TAU/MAPT. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
Tissue Specificity:	Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
BioGrid:	110308. 118 interactions.