Polyclonal Antibody to MECP2
Figure-1: Immunofluorescent analysis of rat cerebellum section costained with rabbit pAb to MeCP2,(34-1063), dilution 1:5,000, in red, and chicken pAb to calbindin,(34-1020), dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells to a variable degree. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.
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Format : | Purified |
Amount : | 100 µl |
Isotype : | Rabbit, IgG |
Content : | Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:10,000-1:20,000 IF/ICC or IHC: 1:2,000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Nucleus |
Post transnational modification: | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
Tissue Specificity: | Present in all adult somatic tissues tested. |
BioGrid: | 110368. 174 interactions. |
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