Polyclonal Antibody to MECP2

Name: Polyclonal Antibody to MECP2
Brand: Abeomics
SKU: 34-1063
Price: 462.0000 USD
Availability: InStock
Rating: 4.5 (10 reviews)

Figure-1: Immunofluorescent analysis of rat cerebellum section costained with rabbit pAb to MeCP2,(34-1063), dilution 1:5,000, in red, and chicken pAb to calbindin,(34-1020), dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells to a variable degree. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.

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Figure-1: Immunofluorescent analysis of rat cerebellum section costained with rabbit pAb to MeCP2,(34-1063), dilution 1:5,000, in red, and chicken pAb to calbindin,(34-1020), dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45Î¼M, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells to a variable degree. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.

$Figure-2: Western blot analysis of tissue and cell lysates using rabbit pAb to MeCP2,(34-1063), dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa$

Product code: 34-1063

Clonality :	Polyclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human, monkey, Rat, Mouse

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Rabbit, IgG
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	MECP2
Gene ID :	4204
Uniprot ID :	P51608
Immunogen Information :	Synthetic peptides from aa 471-486 of human MeCP2

WB: 1:10,000-1:20,000 IF/ICC or IHC: 1:2,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Nucleus
Post transnational modification:	Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Tissue Specificity:	Present in all adult somatic tissues tested.
BioGrid:	110368. 174 interactions.