Polyclonal Antibody to HSP27 (Phospho-Ser15)
Figure 1: Western blot analysis of extracts from Hela cells untreated or treated with UV using HSP27(Phospho-Ser15) Antibody 35-1157 .
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Involved in stress resistance and actin organization.
Tremolada L, et al. (2005) Proteomics. 5(3): 788-795.
McGregor E, et al. (2004) Mol Cell Proteomics. 3(2): 115-124.
Benn SC, et al. (2002) Neuron. 36(1): 45-56.
MacDonald JA, et al. (2002) Mol Cell Proteomics. 1(4): 314-322.
Predicted MW: 27kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Cytoplasm |
| Post transnational modification: | Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925). |
| Tissue Specificity: | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
| BioGrid: | 109547. 439 interactions. |
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