Polyclonal Antibody to Heat shock protein 27, HSP27

Name: Polyclonal Antibody to Heat shock protein 27, HSP27
Brand: Abeomics
SKU: 34-1049
Price: 462.0000 USD
Availability: InStock
Rating: 5 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cells stained with chicken pAb to HSP27,(34-1049), dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. The (34-1049) antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.

Polyclonal Antibody to Heat shock protein 27, HSP27

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Figure-1: Immunofluorescent analysis of HeLa cells stained with chicken pAb to HSP27,(34-1049), dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. The (34-1049) antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.

Figure-2: Western blot analysis of whole cell lysates using chicken pAb to HSP27,(34-1049), dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Î²-tubulin,(34-1116), dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Î²-tubulin proteins.

Product code: 34-1049

Clonality :	Polyclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human, unreactive with rodent

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Conc. IgY prep.
Amount :	100 µl
Isotype :	Chicken, IgY
Content :	Antibody is supplied as an aliquot of concentrated IgY preparation.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	HSBP1
Gene ID :	3315
Uniprot ID :	P04792
Alternative Name :	28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP 27, Heat shock 27 kDa protein, SRP27, Stress-responsive protein 27
Immunogen Information :	Recombinant full length purified HSP27 from E. coli

WB: 1:2,000-5,000. IF/ICC and IHC:1:1,000

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Nucleus, Cytoplasm
Post transnational modification:	Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).
Tissue Specificity:	Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
BioGrid:	109547. 439 interactions.