Polyclonal Antibody to Glyceraldehyde 3-Phosphate Dehydrogenase
Figure-1: Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH,(34-1048), dilution 1:2,000 in red. Blue is Hoescht staining of nuclear DNA. The (34-1048) antibody produces diffuse cytoplasmic staining of cells.
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Shipping Info:
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| Format : | Sera |
| Amount : | 100 µl |
| Isotype : | Rabbit, IgG |
| Content : | Antibody is supplied as an aliquot of serum |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:30,000 IF/ICC and IHC: 1:2,000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Cytoplasm, Membrane, Cytoplasm |
| Post transnational modification: | Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation. |
| BioGrid: | 108868. 233 interactions. |
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