Polyclonal Antibody to E2F-1
Figure 1: Western blot analysis of extracts from 3T3 cells using E2F-1 Antibody 35-1814 .
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Rabbit IgG |
| Content : | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. "P53 and E2F-1 cooperate to mediate apoptosis." Wu X., Levine A.J. Proc. Natl. Acad. Sci. U.S.A. 91:3602-3606(1994) "Regulation of E2F1 activity by acetylation." Martinez-Balbas M.A., Bauer U.M., Nielsen S.J., Brehm A., Kouzarides T. EMBO J. 19:662-671(2000) "Regulation of E2F1 function by the nuclear corepressor KAP1." Wang C., Rauscher F.J. III, Cress W.D., Chen J. J. Biol. Chem. 282:29902-29909(2007)
Predicted MW: 70kd, Western blotting: 1:500~1:1000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus |
| Post transnational modification: | Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. |
| BioGrid: | 108201. 121 interactions. |
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