Polyclonal Antibody to ASAP3
Fig-1: Western blot analysis of ASAP3. Anti-ASAP3 antibody (11-8066) was used at 4 µg/ml on Pancrease lysate.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
ASAP3 belongs to Arf-specific GTPase-activating proteins which regulate Arfs by stimulating their intrinsic GTP hydrolysis. This protein contains a catalytic core of a PH (Pleckstrin Homology) domain, a GAP motif, and two ANK (Ankyrin) repeats. ASAP3 also associates with focal adhesions and with cytoplasmic membranes at the circular dorsal ruffles, where the actin skeleton is remodeled. Depletion of ASAP3 by RNA interference suppresses cell migration in wound healing.
Western blot analysis: 2-4 µg/ml
This antibody detects ~43 kDa band. The size corresponds to Isoform 2 (Uniprot ID-Q8TDY4-2) of ASAP3, the right size is ~99 kDa.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Tissue Specificity: | Highly expressed in primary hepatocarcinoma. Detected in lung, liver and blood leukocytes. |
| BioGrid: | 120756. 15 interactions. |
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