Polyclonal Antibody to ASAP2
Fig-1: Western blot analysis of ASAP2. Anti-ASAP2 antibody (11-8068) was used at 4 µg/ml on Pancrease lysate.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
ASAP2 (Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2) or Development and differentiation-enhancing factor 2 (DDEF2) or Paxillin-associated protein with ARF GAP activity 3 (PAG3) is a multidomain protein containing two ANK repeats, one Arf-GAP domain, one PH domain and one SH3 domain. ASAP2 has two isoforms: PAPalpha and PAPbeta (111 and 106 kDa respectively) and both the isoforms are generally located in Golgi plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). ASAP2 regulates ARF-mediated vesicle budding when vesicular transport. ASAP2 has high level of expression in brain, kidney, and heart.
Western blot analysis: 2-4 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Golgi apparatus, Cell membrane |
| Post transnational modification: | Phosphorylated on tyrosine residues by SRC and PTK2B. |
| Tissue Specificity: | Detected in heart, brain, placenta, kidney, monocytes and pancreas. |
| BioGrid: | 114378. 18 interactions. |
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