Anti-Sonic Hedgehog Polyclonal Antibody
Figure 1: Anti-Sonic Hedgehog antibody(39-2057). Western blotting : Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Intestine Tissue Lysate, Lane 3: HELA Cell Lysate, Lane 4: A549 Cell Lysate, Lane 5: SMMC Cell Lysate, Lane 6: MM231 Cell Lysate.
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| Amount : | 100 μg/vial |
| Isotype : | Rabbit IgG |
| Purification : | Immunogen affinity purified. |
| Content : | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitute : Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
Western blot : 0.1-0.5μg/ml; Immunohistochemistry(Paraffin-embedded Section) : 0.5-1μg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane |
| Post transnational modification: | Sonic hedgehog protein N-product: The lipidated N- and C-terminal peptides of ShhNp can be cleaved (shedding)(PubMed:24522195). The N-terminal palmitoylated peptide is cleaved at the Cardin-Weintraub (CW) motif site (PubMed:24522195). The cleavage reduced the interactions with heparan sulfate. The cleavage is enhanced by SCUBE2 (PubMed:24522195, PubMed:23118222). |
| BioGrid: | 112365. 8 interactions. |
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