Phospho-CrkL (Tyr207) (Clone: G4) rabbit mAb APC conjugate
Fig-1: Flow cytometric analysis of K562 cells treated with imatinib and unstained (blue) as negative control or treated with imatinib (red) or treated with pervanadate (green) and stained using Phospho-CrkL (Tyr207) APC conjugated antibody CrkLY207-G4.
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| Format : | Conjugated |
| Amount : | 100 Tests |
| Isotype : | Rabbit IgG1k |
| Content : | 1X PBS, 0.09% NaN3, 0.2% BSA |
| Storage condition : | Store at 2-8°C. Avoid repeated freeze and thaw cycles. |
| Alternative Name : | Crk-like protein; CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like |
| Immunogen Information : | A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human phospho CrkL |
CrkL (v-Crk sarcoma virus CT10 oncogene-like protein) is an adaptor protein composed of one Src Homology 2 (SH2) and two Src Homology 3 (SH3) domains separated by flexible linker sequences that act as building blocks to assemble multiprotein complexes (1). The Crk adaptor proteins (Crk and CrkL) constitute an integral part of a network of essential signal transduction pathways in humans and other organisms that act as major convergence points in tyrosine kinase signaling. CRKL is required for the normal development of multiple tissues that rely on fibroblast growth factor 8 (FGF8). Phosphorylation of Crk on Tyr 221 or CrkL on Tyr 207 causes intramolecular binding of the linker region to the SH2 domain, sequestering the SH2 and SH3N and preventing them from binding target proteins (2,3). Mounting evidence indicates that dysregulation of Crk proteins is associated with human diseases, including cancer and susceptibility to pathogen infections.
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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