Mouse Monoclonal Antibody to ZAP70 (Clone: 1475CT217.34.65)(Discontinued)
Figure 1: Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with ZAP70 Antibody (10-6628) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Format : | Purified |
Amount : | 400 µl |
Isotype : | Mouse IgG1,Kappa |
Purification : | Protein G Chromatography |
Content : | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
Storage condition : | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles. |
FACS~1:25||WB~1:4000 ||IF~1:25
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm, Cell membrane |
Post transnational modification: | Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B. |
Tissue Specificity: | Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells. |
BioGrid: | 113367. 52 interactions. |
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