Mouse Monoclonal Antibody to NGFR (Clone: 8G3G10)(Discontinued)
Figure 1: Fluorescent confocal image of SY5Y cells stained with NGFR antibody (10-6534). SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with NGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
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| Format : | Purified |
| Amount : | 400 ul |
| Isotype : | Mouse IgG2b,Kappa |
| Purification : | Protein G Chromatography |
| Content : | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| Storage condition : | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
IF~1:100|| WB~1:500~1000|| IHC-P~1:50~100
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Membrane |
| Post transnational modification: | Phosphorylated on serine residues. |
| BioGrid: | 110870. 37 interactions. |
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