Mouse Monoclonal Antibody to NGFR (Clone: 8G3G10)(Discontinued)

Name: Mouse Monoclonal Antibody to NGFR (Clone: 8G3G10)(Discontinued)
Brand: Abeomics
SKU: 10-6534
Price: 472.0000 USD
Availability: InStock
Rating: 5 (10 reviews)

Figure 1: Fluorescent confocal image of SY5Y cells stained with NGFR antibody (10-6534). SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with NGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 Î¼g/ml, 5 min).

Mouse Monoclonal Antibody to NGFR (Clone: 8G3G10)(Discontinued)

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Figure 1: Fluorescent confocal image of SY5Y cells stained with NGFR antibody (10-6534). SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with NGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 Î¼g/ml, 5 min).

Figure 2: Western blot analysis of NGFR Antibody (10-6534) in mouse heart tissue lysates (35Î¼g/lane). NGFR was detected using the purified Mab.(1:1000)

Figure 3: Immunohistochemistry analysis of NGFR Antibody (10-6534) in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGFR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Product code: 10-6534

Clone name :	8G3G10
Clonality :	Monoclonal
Application :	IF, WB, IHC-P
Reactivity :	Human, Mouse

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	400 ul
Isotype :	Mouse IgG2b,Kappa
Purification :	Protein G Chromatography
Content :	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage condition :	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles.


Gene :	NGFR
Gene ID :	4804
Uniprot ID :	P08138
Alternative Name :	Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, p75 ICD, CD271, NGFR, TNFRSF16

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

IF~1:100|| WB~1:500~1000|| IHC-P~1:50~100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.