Mouse Monoclonal Antibody to Mouse CTLA-4 (Clone : MCT.F6) (Discontinued)
Figure-1 : ELISA binding of CTLA4 antibody (Clone: MCT.F6) with mouse CTLA4 recombinant protein, Coating antigen: CTLA4-Fc at 1 µg/ml, CTLA4 antibody dilution start from 1000 ng/ml, EC50= 5.034 ng/ml.
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Mouse IgG1, Kappa |
| Purification : | Protein A chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), also known as CD152, is one of the first well studied immune checkpoint molecules. CTLA4 is expressed on T cells and its expression level can be up-regulated after T cell activation. CTLA4 has two ligands CD80 (B7-1) and CD86 (B7-2). CTLA4 can compete with CD28 binding with these two ligands and transmits an inhibitory signal to T cells. Therapeutic antibodies against CTLA4 can help to stimulate T cells which can then target many cancer cells. Mouse CTLA-4 antibodies with has blocking effect against CD80/CD86 binding with CTLA4 can serve as a great surrogate antibody in mouse model. Anti-Mouse CTLA-4 Antibody (MCT.F6), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant mouse CTLA4-Fc
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-15 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane |
| Post transnational modification: | Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Tissue Specificity: | Widely expressed with highest levels in lymphoid tissues. |
| BioGrid: | 198575. 1 interactions. |
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