Mouse Monoclonal Antibody to Human PD-1 (Clone : PD1.D3)
Figure-1 : ELISA binding of human PD-1 antibody (Clone: PD1.D3) with Human PD-1 recombinant protein. Coating antigen: PD-1-Fc, 1µg/ml. PD-1 antibody dilution start from 1000ng/ml, EC50= 12.5 ng/ml.
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Mouse IgG1,Kappa |
| Purification : | Protein A chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Programmed cell death protein 1 (PD-1), is cell surface receptor expressing on T cells and pro-B cells. Binding of its two ligands PD-L1 and PD-L2 could result in down-regulation of the immune system by inhibiting the T-cell activation process. Thus, PD-1 is an important immune checkpoint and popular target for therapeutic antibodies against many cancers. Anti-Human PD-1 Antibody (PD1.D3), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant human PD-1-Fc .
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-15 µg/ml
Flow cytometry: 5-10 µg/ml
Blockade of Receptor-ligand Interaction in Flow cytometry: 5 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Membrane |
| BioGrid: | 111160. 61 interactions. |
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