Mouse Monoclonal Antibody to Human CTLA-4 (Clone : CT.E8) (Discontinued)
Figure-1 : ELISA binding of CTLA4 antibody (Clone: CT.E8) with Human CTLA4 recombinant protein, Coating antigen: CTLA4-Fc at 0.5 µg/ml, CTLA4 antibody dilution start from 10000 ng/ml, EC50= 4.66 ng/ml
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Mouse IgG1, Kappa |
| Purification : | Protein A chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. CTLA-4 is recruited from intracellular vesicles to the immunological synapse beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-15 µg/ml
Flow cytometry: 5-7 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane |
| Post transnational modification: | Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Tissue Specificity: | Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. |
| BioGrid: | 107875. 109 interactions. |
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