Mouse Monoclonal Antibody to CBP Tag (Clone : 1D11E5)
Figure-1 : Western blot analysis of CBP Tag Antibody (Clone: 1D11E5) at 1 µg/ml on CBP-tagged protein (50 & 20ng). IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG was used as secondary Antibody.
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| Format : | Purified |
| Amount : | 40 µg |
| Isotype : | Mouse IgG2b,Kappa |
| Purification : | Protein A chromatography |
| Content : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Storage condition : | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Immunogen Information : | CBP tag peptide conjugated to KLH |
CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins. CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.
Western blot: 1-2 µg/ml.
Immunoprecipitation: 1-2 µg/ml.
ELISA: 0.001-0.002 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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