Mouse Monoclonal Antibody to Beta-actin (Clone: 8H10D10)(Discontinued)

Name: Mouse Monoclonal Antibody to Beta-actin (Clone: 8H10D10)(Discontinued)
Brand: Abeomics
SKU: 10-6508
Price: 472.0000 USD
Availability: InStock
Rating: 5 (10 reviews)

Figure 1: Western blot analysis of Beta-actin Antibody (10-6508) in lane 1: HepG2 cell line and lane 2: HL-60 cell line. Beta-actin Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20Î¼g per lane.

Mouse Monoclonal Antibody to Beta-actin (Clone: 8H10D10)(Discontinued)

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Figure 1: Western blot analysis of Beta-actin Antibody (10-6508) in lane 1: HepG2 cell line and lane 2: HL-60 cell line. Beta-actin Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20Î¼g per lane.

Figure 2: Immunohistochemical analysis of paraffin-embedded h spleen section using Beta-actin Antibody (10-6508). Beta-actin Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Figure 3: Western blot analysis of Beta-actin Antibody (10-6508) in lane 1: HepG2 cell line lane 2: HL-60 cell line, lane 3: mouse NIH/3T3 cell line, lane 4: mouse cerebellum, lane 5: rat stomach tissue lysate, lane 6: CHO and lane 7: COS-7 cell line. Beta-actin Antibody was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35Î¼g per lane.

Figure 4: Immunohistochemical analysis of paraffin-embedded h spleen section using Beta-actin Antibody (10-6508). Beta-actin Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Product code: 10-6508

Clone name :	8H10D10
Clonality :	Monoclonal
Application :	WB, IHC-P
Reactivity :	Human, Mouse, Rat

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	400 µl
Isotype :	Mouse IgG
Purification :	Protein G Chromatography
Content :	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage condition :	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term store at -20°C in small aliquots to prevent freeze-thaw cycles.


Gene :	ACTB
Gene ID :	60
Uniprot ID :	P60709
Alternative Name :	Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

WB~1:1000|| IHC-P~1:25

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Nucleus
Post transnational modification:	(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
BioGrid:	106575. 336 interactions.