Monoclonal Antibody to Beta Tubulin(Clone: 4E4)
Figure-1: Immunofluorescence analysis of HeLa cells stained with mouse mAb to β-tubulin,(34-1117), dilution 1:5,000 in green, and costained with chicken pAb to lamin A/C,(34-1057), dilution 1:2,000, in red. Blue is DAPI staining of nuclear DNA. (34-1117) antibody produces strong staining of cytoplasmic microtubules, while the lamin A/C antibody specifically labels the nuclear membrane of these cells.
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Shipping Info:
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Mouse, IgG2a |
| Content : | Antibody is supplied as an aliquot of affinity purified antibody. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:5,000-1:10,000, ICC/IF and IHC: 1:1000-1:5,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Post transnational modification: | Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Tissue Specificity: | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
| BioGrid: | 128444. 265 interactions. |
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