Monoclonal Antibody to Beta Tubulin(Clone: 1B12)
Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to β-tubulin,(34-1116), dilution 1:5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. (34-1116) antibody produces strong staining of microtubules in the cytoplasm, while the chicken pAb to the nucleic acid binding protein FOX2 antibody labels the nuclei of HeLa cells. Mouse select each image for larger view.
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Shipping Info:
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Format : | Purified |
Amount : | 100 µl |
Isotype : | Mouse, IgG2b |
Content : | Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:5,000-1:10,000. IF: 1:5,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm |
Post transnational modification: | Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
Tissue Specificity: | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
BioGrid: | 128444. 265 interactions. |
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