Monoclonal Antibody to Lamp1 (Clone: 5H6)

Name: Monoclonal Antibody to Lamp1 (Clone: 5H6)
Brand: Abeomics
SKU: 34-1060
Price: 462.0000 USD
Availability: InStock
Rating: 4.8 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1,(34-1060), dilution 1:500 in red, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The cells were treated with 50µM of chloroquine, an inhibitor of autophagy, for 16 hours prior to staining. The (34-1060) antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the(34-1126) antibody specifically labels the intermediate filament network in these cells.

Monoclonal Antibody to Lamp1 (Clone: 5H6)

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Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1,(34-1060), dilution 1:500 in red, and costained with chicken pAb to vimentin,(34-1126), dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The cells were treated with 50µM of chloroquine, an inhibitor of autophagy, for 16 hours prior to staining. The (34-1060) antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the(34-1126) antibody specifically labels the intermediate filament network in these cells.

Figure-2: Western blot analysis of different cell lysates using mouse mAb to LAMP1,(34-1060) dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody does not recognize the rodent LAMP1 homologue. The same blot was probed with chicken pAB to HSP60, dilution 1:20,000. The HSP60 antibody reveals heat shock 60 protein with apparent molecular weight of 60kDa in all preparations.

Product code: 34-1060

Clone name :	5H6
Clonality :	Monoclonal
Application :	WB, IF/ICC, IHC
Reactivity :	Human specific

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody or concentrated tissue culture supernatant.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	LAMP1
Gene ID :	3916
Uniprot ID :	P11279
Alternative Name :	CD107 antigen-like family member A
Immunogen Information :	Full length human Lamp1 purified from E. coli

WB: 1:10,000. IF/ICC and IHC: 1:2,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Endosome membrane, Lysosome membrane, Late endosome
Post transnational modification:	O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.
BioGrid:	110110. 63 interactions.