Monoclonal Antibody to Heat shock protein 27, HSP27(Clone: 6H11)

Name: Monoclonal Antibody to Heat shock protein 27, HSP27(Clone: 6H11)
Brand: Abeomics
SKU: 34-1050
Price: 462.0000 USD
Availability: InStock
Rating: 5 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to HSP27,(34-1050), dilution 1:2,000 in green, and costained with chicken pAb to HSP60, dilution 1:2,000 in red. Blue is DAPI staining of nuclear DNA. The (34-1050) antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria.

Monoclonal Antibody to Heat shock protein 27, HSP27(Clone: 6H11)

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Figure-1: Immunofluorescent analysis of HeLa cells stained with mouse mAb to HSP27,(34-1050), dilution 1:2,000 in green, and costained with chicken pAb to HSP60, dilution 1:2,000 in red. Blue is DAPI staining of nuclear DNA. The (34-1050) antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria.

Figure-2: Western blot analysis of different tissue or whole cell lysates using mouse mAb to HSP27,(34-1050), dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. Strong single band at ~27kDa corresponds to HSP27 protein, detected only in human cell lines, since this antibody is human specific. The blot was simultaneously probed with rabbit pAb to HSP60, dilution 1:5,000 in red. A strong 60kDa band is present in all preparations.

Product code: 34-1050

Clone name :	6H11
Clonality :	Monoclonal
Application :	WB, ICC/IF, IHC
Reactivity :	Human, unreactive on rodent

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	100 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody or concentrated tissue culture supernatant.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	HSPB1
Gene ID :	3315
Uniprot ID :	P04792
Alternative Name :	28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP 27, Heat shock 27 kDa protein, SRP27, Stress-responsive protein 27
Immunogen Information :	Recombinant full length purified HSP27 from E. coli.

WB: 1:10,000. IF/ICC and IHC: 1:1,000.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Nucleus, Cytoplasm
Post transnational modification:	Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).
Tissue Specificity:	Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
BioGrid:	109547. 439 interactions.