Monoclonal Antibody to Ep-CAM / CD326 (Rat) (Epithelial Marker)(Clone : SPM534)
Western Blot of rat Colon Lysate probed with Ep-CAM Monoclonal Antibody (SPM534).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Purification : | Affinity Chromatography |
| Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
Flow Cytometry (5-10ul/million cells); Immunofluorescence (1:50-1:100); Western Blot (1:100-1:200); Immunoprecipitation (5-10ul/500ug protein lysate);Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT)(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C)
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Lateral cell membrane, Cell junction |
| Post transnational modification: | Glycosylation at Asn-198 is crucial for protein stability. |
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