Monoclonal Antibody to EMI1 (Early Mitotic Inhibitor-1)(Clone : EMI1/1176)

Product code: 36-1169

Clone name : EMI1/1176
Clonality : Monoclonal
Application : WB, IHC
Reactivity : Human

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100 µg
$520.00 

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG2a, kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : FBXO5
Gene ID : 26271
Uniprot ID : Q9UKT4
Alternative Name : FBXO5, EMI1, FBX5
Immunogen Information : Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Western Blot (1-2ug/ml for 60 minutes at RT);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Nucleus, Cytoplasm, Cytoplasm
Post transnational modification: Ubiquitinated by the by the SCF(BTRC) complex following phosphorylation by PLK1.
BioGrid: 117655. 38 interactions.
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