Monoclonal Antibody to Cytokeratin, Multi (Epithelial Marker)(Clone : KRT/457)(Discontinued)
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1 |
| Purification : | Affinity Chromatography |
| Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1.0µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistology (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Tissue Specificity: | Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. |
| BioGrid: | 110051. 45 interactions. |
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