Monoclonal Antibody to Cytokeratin 8/18(Clone : SPM141)(Discontinued)

Product code: 36-11112

Clone name : SPM141
Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1 Kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : KRT8
Gene ID : 3856
Uniprot ID : P05787
Alternative Name : KRT8, CYK8
Immunogen Information : KeRatin prepaRation from human breast carcinoma MCF-7 cells
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistology (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cytoplasm, Nucleus, Nucleus matrix
Post transnational modification: O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Tissue Specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
BioGrid: 110054. 69 interactions.
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