Monoclonal Antibody to Biotin (Vitamin B7 or Vitamin H)(Clone : SPM375)
Formalin-fixed, paraffin-embedded human Tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin Monoclonal Antibody (SPM375).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Purification : | Affinity Chromatography |
| Content : | 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
| Immunogen Information : | Biotinylated sheep immunoglobulin |
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Confocal Microscopy; Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); In situ Nucleic Acid Hybridization; Western Blot; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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