Monoclonal Antibody to AKT1 (Clone: ABM12F7)
Fig-1: Western blot analysis of AKT1. Anti- AKT1 antibody (Clone: ABM12F7) was tested at 2 µg/ml on SKBR3, PC3 and BT474.
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Shipping Info:
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1 Kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Western blot analysis: 2-4 µg/ml, Flowcytometric analysis: 0.5-1µg/10^6 cells
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane |
| Post transnational modification: | Acetylated on Lys-14 and Lys-20 by the histone acetyltransferases EP300 and KAT2B. Acetylation results in reduced phosphorylation and inhibition of activity. Deacetylated at Lys-14 and Lys-20 by SIRT1. SIRT1-mediated deacetylation relieves the inhibition. |
| Tissue Specificity: | Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. |
| BioGrid: | 106710. 302 interactions. |
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