Monoclonal Antibody to Actin, pan-specific (Clone: 5J11)
Figure-1: Immunofluorescent analysis of HeLa cells stained with mAb against actin,(34-1002), in green, costained with chicken pAb against vimentin,(34-1126), in red. The actin antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. The vimentin antibody stains a different cytoskeletal network, the intermediate or 10nm filaments. The blue is DAPI staining of nuclear DNA.
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Shipping Info:
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| Format : | Purified |
| Amount : | 100 µl |
| Isotype : | Mouse, IgG1 |
| Content : | Supplied as an aliquot of affinity purified antibody at 1mg/ml in PBS, 50% glycerol, 5mM NaN3 |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:1,000. IF/ICC or IHC: 1:500-1,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Post transnational modification: | (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
| BioGrid: | 106573. 145 interactions. |
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