IL-1beta (human) (Recombinant) (untagged)
Figure 1: Activation of NF-kB in HeLa cells with IL-1 beta (human) (rec.) (untagged) . Method: Cell extracts from Hela cells treated with differerent concentrations of IL-1 beta (human) (rec.)(untagged) for 30 min (A) or with 50ng/ml of IL-1 beta (human) (rec.) (untagged) for different time points (B) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-Ikb or anti-phospho IkB (1/1000) (CST) to measure IkB degradation (A) or IkB phosphorylation and degradation (B).
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| Amount : | 10 µg |
| Purification : | >=95% (SDS-PAGE) |
| Content : | Reconstitute with 100µl sterile water.0.1mg/ml after reconstitution. Lyophilized. Contains PBS. |
| Storage condition : | Short Term Storage +4°C ; Long Term Storage-20°C ;After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0. 1% BSA should be used for further dilutions. Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| AA sequence : | Human IL-1beta (aa 117-269) is untagged. |
| Alternative Name : | Interleukin-1beta; IL-1F2 |
Source :E. coli
Specific Binds to human IL-1R.IL-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta belongs to the IL-1 family of proteins that are involved in the inflammatory response. IL-1beta in most situations requires the inflammasome complex to be cleaved and secreted.
Biological Activity: Activates the NF-kB with an ED50 of <5ng/ml.
Endotoxin Content <0.1EU/µg purified protein (LAL test; Lonza).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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