Anti-Nucleolin (Marker of Human Cells) Monoclonal Antibody(Clone: NCL/902)-CF488

Product code: 36-2842

Clone name : NCL/902
Clonality : Monoclonal
Application : FACS, IF,
Reactivity : Human
Conjugate : CF488

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0.5 ml at 100µg/ml
$520.00 

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Amount : 0.5 ml at 100µg/ml
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous.
Gene : NCL
Gene ID : 4691
Uniprot ID : P19338
Alternative Name : NCL; Nucl; Nucleolin; Protein C23
Immunogen Information : Recombinant human NCL protein
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thoµght to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100);(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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