Anti-mCherry VHH antibody
Figure 1: Indirect ELISA showing anti-mCherry VHH antibody (JOT0076-1) binding to purified mCherry protein. Plates were coated with 200ng/well purified protein and binding of JOT0076-1 assessed in serial dilution from 1ng/ml primary antibody in triplicate.
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| Amount : | 50 µg |
| Isotype : | Camelid VHH |
| Purification : | Affinity chromatography purified via Ni-charged resin. Purity: > 95% as determined by SDS-PAGE |
| Content : | 1 mg/mL by Nanodrop Buffer : 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA, 0.1 % Proclin 300 |
| Storage condition : | 4°C; Do not freeze. |
| Uniprot ID : | X5DSL3 |
Alpaca derived anti-mCherry VHH single domain antibody (molecular weight: 14.7 kDa) with a 6*His tag at its C-terminal, expressed in E. coli under conditions free from animal derived components.
mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel.
This is a product from Jotbody, Hong Kong. This antibody is made available worldwide by ABEOMICS Inc.
Positive controls : Positive ELISA detected in: recombinant fluorescent protein mCherry Positive WB detected in: mCherry-expressing 293T cells Positive IF detected in: mCherry-expressing PLC/PRF/5 cells
Recommended dilutions :ELISA 1:2000-1:10000 WB 1:1000-1:5000 IF 1:500-1:2000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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