Anti-Hu SIGLEC8 PE
Figure 1: Separation of human SIGLEC8 positive eosinophil granulocytes (red-filled) from eosinophil granulocytes (black-dashed) stained using mouse IgG1 isotype control (MOPC-21) PE antibody (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human SIGLEC8 (7C9) APC antibody
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Amount : | 100 Tests |
Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Content : | Concentration: 1 mg/ml Storage Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.
Specificity :The mouse monoclonal antibody 7C9 recognizes an extracellular epitope of SIGLEC8, an eosinophil marker, expressed e.g. in lymph nodes and spleen.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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