Anti-HLA-E APC Mab (3D12)
Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA-E (3D12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
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Amount : | 0.1 mg |
Isotype : | Mouse IgG1 |
Purification : | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Content : | Concentration: 0.1 mg/ml Storage Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Specificity: The mouse monoclonal antibody 3D12 (also known as 3D12HLA-E) recognizes native extracellular part of HLA-E, an ubiquitously expressed non-classical MHC class I molecule, as well as free HLA-E.
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
Flow cytometry: Recommended dilution: 1-4 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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