Anti-beta2-Microglobulin Monoclonal Antibody (Clone:B2M-01)

Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Western blotting: Recommended dilution: 2-4 μg/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli. <br>Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. <br>ELISA: Working dilution should be determinated by investigator. <br>RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10^-8 mol/l as determined by competitive RIA. <br>Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.