Anti-beta2-Microglobulin Monoclonal Antibody (Clone:B2M-01)
Fig. 1: Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line. Lane 2: EL4 mouse lymphoblastic lymphoma cell line. Lane 3: U937 human histiocytic lymphoma cell line
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG2a |
| Purification : | Purified by precipitation methods |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Western blotting: Recommended dilution: 2-4 μg/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. <br>Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli. <br>Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. <br>ELISA: Working dilution should be determinated by investigator. <br>RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10<sup>-8</sup> mol/l as determined by competitive RIA. <br>Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Post transnational modification: | Glycation of Ile-21 is observed in long-term hemodialysis patients. |
| BioGrid: | 107044. 43 interactions. |
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