TNF-Alpha, Soluble (human) (rec.)
Figure 1: TNF-Alpha, Soluble (human) (rec.) induces NF-kB dependent genes. Methods: Endothelial cells (HUVEC) are incubated for 1h in the presence of BAY 11-7082 and then treated with TNFAlpha (20ng/ml) (red bar) or incubated with TNFAlpha (20ng/ml) (in the presence or absence of TNF-R1 (human):Fc (human) (rec.) (0.5µg/ml) (blue and gray bar, respectively). TNFAlpha-induced genes expression such as E-Selectin or MCP-1 are tested by flow cytometry.
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| Amount : | 50 µg |
| Purification : | >=95% (SDS-PAGE) |
| Content : | Reconstitution 10 µg size: Reconstitute with 100 µl sterile water. 50 µg size: Reconstitute with 50 µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. Concentration 10 µg size: 0.1 mg/ml after reconstitution. 50 µg size: 1 mg/ml after reconstitution. Formulation: Lyophilized. Contains PBS. |
| Storage condition : | Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| AA sequence : | A FLAG®-tag is fused at the N-terminus of human TNF-Alpha (aa 85-223). |
| Alternative Name : | Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin |
Source : E. coli
Binds to human and mouse TNF-R1.Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-Alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-Alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-Alpha (MultimericTNF-Alpha). Since the MultimericTNF-Alpha mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-Alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-Alpha or MultimericTNF-Alpha can be used.
MW :~17kDa (SDS-PAGE); Biological Activity:Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells). Endotoxin Content <0.01EU/µg purified protein (LAL test; Lonza).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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