TLR4/HEK293 Stable Cell Line
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Fig-1: Functional analysis of the TLR4/HEK293 cell line. TLR4/HEK293 cells as well as parental HEK293 cells were transfected with CD14, MD-2 and NF-kB/Renilla luciferase reporter plasmids for 16 h. Cells were stimulated with different doses of LPS (Abeomics, Cat. #15-1013) for 16 h followed by luciferase assay using Luciferase Assay Reagent (Abeomics, Cat. #17-1101).
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Shipping Info:
Order now and get it on Tuesday September 03, 2024
Same day delivery FREE on San Diego area orders placed by 1.00 PM
Amount : | 1 Vial |
Content : | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO |
Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
TLR4/HEK293 Stable Cell Line is a stably transfected HEK293 cell line which expresses human Toll-like receptor 4 (TLR4, also designated as CD284). TLR4 mediates immune responses by recognition of lipoplysaccharides (LPS) with the help of LPS-binding protein, CD14 and MD-2. TLR4 stimulation uniquely triggers two signaling pathways named the MyD88-dependent pathway and the TRIF-dependent pathway, which lead to the production of two sets of cytokines such as proinflammatory cytokines and type I interferons/chemokines, respectively. Note that TLR4 in the TLR4/HEK293 stable cell line contains the N-terminal HA tag (Figure 2), which does not interfere with TLR4 activity as confirmed by functional assay (Figure 1).
Sequence data: Human TLR4 (accession number NP_612564)
Application:.
- Functional assay.
Culture conditions:
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research. The buyer cannot sell, give or otherwise transfer this product to a third party.
Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics ([email protected]).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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