TCF/LEF Leeporter™ Luciferase Reporter-HEK293 Cell Line

Fig-1: Induction of TCF/LEF activity by LiCl in TCF/LEF Leeporter™ - HEK293 cells.

TCF/LEF Leeporter™ Luciferase Reporter-HEK293 Cell Line

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Fig-1: Induction of TCF/LEF activity by LiCl in TCF/LEF Leeporter™ - HEK293 cells.

Fig-2: Induction of TCF/LEF activity by Wnt3a in TCF/LEF Leeporter™ - HEK293 cells (Wnt3a activity assay was performed in the presence of 10 mM LiCl).

Fig-3: Inhibition of Wnt3a-induced TCF/LEF activity by IWR-1 in TCF/LEF Leeporter™ - HEK293 cells (Assay was performed in the presence of 10 mM LiCl).

Product code: 14-119ACL

Application :	Functional Assay

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Shipping Info:

Order now and get it on Friday November 22, 2024

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Download TDS / Manual MSDS

Amount :	1 Vial
Content :	Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage condition :	Immediately upon receipt, store in liquid nitrogen.

The TCF/LEF Leeporter™ Luciferase Reporter cell line is a stably transfected HEK293 cell line which expresses Renilla luciferase reporter gene under the control of the TCF/LEF response element. This cell line is designed to monitor the transcriptional activity of TCF/LEF and can be used for studying Wnt signaling pathways as well as screening of activators or inhibitors that affect the TCF/LEF transcriptional activity.

Application:

Monitor the TCF/LEF induction activity.
Screen for activators or inhibitors of the TCF/LEF signaling pathway.

Culture conditions:

Cells should be grown at 37^oC with 5% CO₂ using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3 µg/ml of Puromycin (Note: Puromycin can be omitted during the reporter cell assays).

It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37^oC water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37^oC-CO₂ incubator.

Leave the T25 flask in the incubator for 1~3 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.

To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.

Functional validation:

A. Response of TCF/LEF Leeporter™ – HEK293 cells to lithium chloride (LiCl) (Figure 1).

1. Harvest TCF/LEF Leeporter™ – HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 µl of growth medium at 5 x 10^4 cells/well.

2. Incubate cells at 37^oC in a CO2 incubator for overnight.

3. The next day, stimulate cells with various concentrations of LiCl.

4. Incubate at 37^oC in a CO2 incubator for 16 hours.

5. Equilibrate the plate to room temperature for 10 minutes.

6. Add 50 µl of luciferase assay reagent (Abeomics, Cat #17-1101; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well.

7. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

B. Response of TCF/LEF Leeporter™ – HEK293 cells to Wnt3a (Figure 2).

1. Harvest TCF/LEF Leeporter™ – HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 µl of growth medium at 5 x 10^4 cells/well.

2. Incubate cells at 37^oC in a CO2 incubator for overnight.

3. The next day, stimulate cells with various concentrations of human Wnt3a in the presence of 10 mM LiCl.

4. Incubate at 37^oC in a CO2 incubator for 16 hours.

5. Equilibrate the plate to room temperature for 10 minutes.

6. Add 50 µl of luciferase assay reagent (Abeomics, Cat #17-1101) per well.

7. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

C. Inhibition of Wnt3a-induced TCF/LEF activity by IWR-1 in TCF/LEF Leeporter™ – HEK293 cells (Figure 3).

1. Harvest TCF/LEF Leeporter™ – HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 µl of growth medium at 5 x 10^4 cells/well.

2. Incubate cells at 37^oC in a CO2 incubator for overnight.

3. The next day, treat cells with various concentrations of IWR-1 in the presence of 250 ng/ml Wnt3a plus 10 mM LiCl.

4. Incubate cells at 37^oC in a CO2 incubator for 16 hours.

5. Equilibrate the plate to room temperature for 10 minutes.

6. Add 50 µl of luciferase assay reagent (Abeomics, Cat #17-1101) per well.

7. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research. The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics ([email protected]).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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