TaqDNA Recombinant Protein
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Amount : | 3000U |
Purification : | Greater than 95.0% as determined by SDS-PAGE. |
Content : | Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20. |
Storage condition : | Stable for 5 days at 10°C, for longer period of time store at -20°C. |
Alternative Name : | DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1. |
Source : Recombinant e.coli contains Thermus aquaticus polymerase gene. Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
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