Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)
Figure-1: Recombinant COVID-19 Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.
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| Amount : | 100 μg |
| Purification : | >90% by SDS-PAGE. |
| Content : | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
| Storage condition : | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. Avoid repeated freeze/thaw cycles. |
| AA sequence : | The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1fused with a 6×His tag at the C-terminus. |
| Gene ID : | 43740568 |
| Alternative Name : | S1 protein; Spike glycoprotein Subunit1;S glycoprotein Subunit1;Spike protein S1;novel coronavirus s1 Protein |
Source: HEK 293 cells. The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Bio-Activity: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human ACE2 at 2 g/mL (100L/well ) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 μg/mL. Endotoxin : < 1.0 EU/μg of the protein by LAL method.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Paidi RK, Jana M, Raha S, Mishra RK, Jeong B, Sheinin M, Pahan K.
Prenol, but Not Vitamin C, of Fruit Binds to SARS-CoV-2 Spike S1 to Inhibit Viral Entry: Implications for COVID-19.
J Immunol. 2023 May 5:ji2200279. doi: 10.4049/jimmunol.2200279. Epub ahead of print.
PMID: 37144841.
Paidi RK, Jana M, Raha S, McKay M, Sheinin M, Mishra RK, Pahan K.
Eugenol, a Component of Holy Basil (Tulsi) and Common Spice Clove, Inhibits the Interaction Between SARS-CoV-2 Spike S1 and ACE2 to Induce Therapeutic Responses.
J Neuroimmune Pharmacol. 2021 Dec;16(4):743-755. doi: 10.1007/s11481-021-10028-1. Epub 2021 Oct 22.
PMID: 34677731; PMCID: PMC8531902.
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