Recombinant Rabbit Monoclonal Antibody to Phospho-Histone H4 (Ser1), H4S1p (Clone: RM194)(Discontinued)
Figure 1: Western Blot of (1) acid extracts of HeLa cells; (2)recombinant Histone H4. Using Clone: RM194 at 0.5 µg/ml,showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM194 reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighboring Arginine 3 modifications. No cross reactivity with Histone H2A phosphorylated at Serine 1. Western Blot: 0.5 µg/ml - 2 µg/ml; ICC: 1 µg/ml - 2 µg/ml; ELISA: 0.2 µg/ml - 1 µg/ml; Multiplex: 0.1 µg/ml - 1 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome |
| Post transnational modification: | Butyrylation of histones marks active promoters and competes with histone acetylation. |
| BioGrid: | 299853. 18 interactions. |
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