Recombinant Rabbit Monoclonal Antibody to Phospho-Histone H2B (Ser14) (Clone: RM238)(Discontinued)
Figure 1: Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using Clone: RM238 at 0.5 µg/ml, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM238 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones. Western Blot: 0.5 µg/ml - 2 µg/ml; ICC: 1 µg/ml - 2 µg/ml; ELISA: 0.2 µg/ml - 1 µg/ml; Multiplex: 0.1 µg/ml - 1 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Chromosome |
| Post transnational modification: | Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. |
| BioGrid: | 109271. 62 interactions. |
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